Thursday, January 26, 2006

HOOF-Prints Help Find Where Outbreaks Begin

Locating potential sources of brucellosis outbreaks is easier now, thanks to a new DNA fingerprinting technique developed by scientists with the Agricultural Research Service (ARS) and the Animal and Plant Health Inspection Service (APHIS).

Finding the source of these outbreaks helps with identification and isolation of infected animals, and with telling whether the outbreaks started in wildlife, according to microbiologists Betsy Bricker at ARS' National Animal Disease Center and Darla Ewalt of APHIS' National Veterinary Services Laboratories, both in Ames, Iowa.

The new technique--called "HOOF-Prints," for Hypervariable Octameric Oligonucleotide Fingerprints--allows scientists to identify strains of brucellosis through differences in their DNA sequences, and to separate these strains into subtypes.

Brucellosis is an extremely infectious disease caused by Brucella bacteria that induce abortions in many animals, including sheep, goats, cattle, pigs, elk and bison. Humans who come in contact with Brucella can get undulant fever, which is marked by chronic flulike symptoms.

Though almost eradicated from the United States, brucellosis can still prove costly to livestock producers through testing and losses. Outbreaks may cause states to lose brucellosis-free status, meaning their cattle must undergo extensive testing before they can be shipped away.

The new method uses polymerase chain reaction (PCR) technology, which copies large amounts of DNA molecules from small amounts of source DNA.

According to Ewalt, the HOOF-Prints technique is intended to complement existing PCR and bacteriological tests used to identify Brucella species.HOOF-Prints was first applied in the field in 2002 when it was used to trace a brucellosis outbreak in Fremont County, Idaho, cattle to local elk.

It could eventually be applied toward generating an international database of Brucella fingerprints that would be used to control the disease, according to Bricker.Read more about the research in the January 2006 issue of Agricultural Research magazine, available online at:http://www.ars.usda.gov/is/AR/archive/jan06/hoof0106.htm

***************************************************************************************************************

AGRI-INFO/JANUARY 26, 2006



TITLE: HOOF-PRINTS HELP FIND WHERE OUTBREAKS BEGIN


ALAM BA NINYO NA MAS MADALI NA NGAYON PARA MATUKOY ANG PINAGMULAN NG BRUCELLOSIS BATAY SA BAGONG DNA FINGERPRINTING TECHNIQUE NA NATUKLASAN NG SIYENTISTA NG ARS AT US ANIMAL AND PLANT HEALTH INSPECTION SERVICE?

AYON KAY MICROBIOLOGIST BETSY BRICKER, ANG PAG-BULL’S EYE SA SOURCE NG OUTBREAK NG BRUCELLOSIS AY MAKATUTULONG NG MALAKI SA PAGTUKOY AT PAGWALAY SA MGA INFECTED NA ALAGANG HAYOP, AT MALALAMAN DIN KUNG ITO AY NAGMULA SA WILDLIFE.

ANG BAGONG TECHNIQUE NA ITO AY TINAWAG SA PANGALANG “HOOF-PRINTS” O ANG HYPERVARIABLE OCTAMERIC OLIGONUCLEOTIDE FINGERPRINTS.

ANG GANITONG PAMAMARAAN AY MAKATUTULONG NG MALAKI PARA MATUKOY ANG STRAINS NG BRUCELOSIS SA PAMAMAGITAN NG PAGKAKAIBA NG KANILANG DNA SEQUENCE AT IHIWALAY ANG MGA STRAIN NA ITO SA SUB TYPES.

ANG BRUCELLOSIS AY ISANG NAPAKATINDING INFECTIOUS DISEASE NA SANHI NG BRUCELLA BACTERIA NA NAGRERESULTA SA ABORTION SA MGA ALAGANG HAYOP TULAD NG TUPA, KAMBING, BAKA, BABOY, ELK AT BISON.

0 Comments:

Post a Comment

<< Home