Wednesday, May 24, 2006

Precision Biochemistry Tracks DNA Damage In Fish

Like coal-mine canaries, fish DNA can serve as a measure of the biological impact of water and sediment pollution--or pollution clean-up.

That's one of the conclusions of a new study* by researchers from the Pacific Northwest Research Institute (PNRI), Woods Hole Oceanographic Institution, the University of Maryland and the National Institute of Standards and Technology (NIST).

Research over the past several years has demonstrated the adverse effects of industrial pollutants in water and sediment on the health of fish in the lower Duwamish River. The Duwamish flows through an industrialized section of south Seattle, Wash., and in 2001 a section of the lower river was added to the Environmental Protection Agency's Superfund list because of contaminants including polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), mercury and other metals, and phthalates. In previous research on Duwamish fish, PNRI used an infrared spectroscopy method to document DNA damage in the gills of English sole.

In a new joint paper, the researchers report on several biomarkers, including pollution-induced P450 enzyme changes, and on infrared spectral analysis of DNA and measurements of specific modifications to DNA from fish gills and livers using liquid and gas chromatography combined with mass spectrometry (LC/MS,GC/MS). Precision chemical analysis techniques at NIST allowed the researchers to identify and measure damage to adenine and guanine, specific chemical components or bases of DNA, at extraordinarily low levels--five lesions out of a 100 million bases in one case. The results correlated well with earlier research and revealed similar damage to liver DNA (more likely tied to the fish's food) and gill DNA (more probably reflecting pollutants in water).

The results suggest that these DNA lesions, and others like them, can be used as very sensitive biomarkers to provide a direct measure of the impact of contaminants on fish populations. Moreover, since natural DNA repair processes may gradually reverse the damage in the absence of further insults, these biomarkers also might be used to help assess the efficacy of pollution remediation efforts. The work was funded in part by the National Institute of Environmental Health Sciences (NIH).

source: http://www.sciencedaily.com/releases/2006/05/060514082623.htm


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AGRI-INFO/MAY 24, 2006



TITLE: PRECISION BIOCHEMISTRY TRACKS DNA DAMAGE IN FISH


ALAM BA NINYO NA SA PAMAMAGITAN NG PRECISION BIOCHEMISTRY AY MATUTUKOY NA KUNG GAANO KATINDI ANG DNA DAMAGE SA ISDA

ANG FISH DNA AY NAGSISILBING SUKATAN NG MGA EKSPERTO SA TINATAWAG NA BIOLOGICAL IMPACT NG TUBIG AT SEDIMENT POLLUTION O POLLUTION CLEAN UP.

ITO AY BATAY SA GINAWANG PAG-AARAL NG MGA EKSPERTO NG PACIFIC NORTHWEST RESEARCH INSTITUTE, WOODS HOLE OCEANOGRAPHIC INSTITUTION, UNIVERSITY OF MARYLAND AT NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY.

GUMAGAMIT ANG MGA EKSPERTO NG INFRARED SPECTROSCOPY METHOD PARA MADOKUMENTO ANG DNA DAMAGE SA HASANG NG ISDA, MAGING NG SA LIVER NITO NA GINAMITAN NAMAN NG LIQUID AT GAS CHROMATOGRAPHY KUMBINASYON NG MASS SPECTROMETRY.

BATAY SA PRECISION CHEMICAL ANALYSIS TECHNIQUES, NATUKOY NG MGA EKSPERTO AT NASUKAT ANG DAMAGE SA ADENINE AT GUANINE, ANG MGA SPECIFIC CHEMICAL COMPONENT O BASES NG DNA SA EXTRAORDINARILY LOW LEVELS.

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