Saturday, June 03, 2006

A Quicker, Cheaper Test for Turkey Viruses

A new, highly sensitive diagnostic test to detect viruses associated with poult enteritis complex, or PEC, has been developed by Agricultural Research Service (ARS) scientists in Athens, Ga.

This disease of young turkeys causes diarrhea, poor weight gain and, in some cases, high mortality.Microbiologists Erica Spackman and Darrell Kapczynski developed the test in a format that allows the detection of several types of viruses at one time. Spackman works in the ARS Endemic Poultry Viral Diseases Research Unit, and Kapczynski is in the Exotic and Emerging Avian Viral Disease Research Unit.

Both units are part of the ARS Southeast Poultry Research Laboratory. The scientists collaborated on the research with Holly Sellers of the University of Georgia.The test relies on a molecular technique called real-time reverse transcription- polymerase chain reaction (RRT-PCR), which is a highly sensitive and specific method for detecting viral RNA.

Current diagnostic methods for PEC-associated viruses have limitations because of poor analytic specificity and sensitivity.The researchers inoculated turkey poults with each of the PEC-associated viruses and later collected intestinal tissue samples and cloacal swabs from the experimentally infected birds.

The RRT-PCR test showed high sensitivity, accurately detecting the target viruses in both the tissues and swabs.While intestinal samples have previously been essential to making a definitive PEC diagnosis, the researchers found that cloacal samples--which are easier to collect and process--were just as suitable for testing.

Using them will not only save time and money, it will also eliminate the need to euthanize birds for sampling.The PEC-test technology has been provided to several laboratories for diagnostic use, and the research team is now working on adapting the RRT-PCR technique to diagnose related diseases in chickens.


************************************************************************************

AGRI-INFO/JUNE 3, 2006



TITLE: A QUICKER, CHEAPER TEST FOR TURKEY VIRUSES


ALAM BA NINYO NA NAGAWANG MAISAKATUPARAN NG MGA EKSPERTO ANG ISANG HIGHLY SENSITIVE DIAGNOSTIC TEST NA NAGLALAYONG MA-DETECT ANG MGA VIRUS NA MAY KAUGNAYAN SA POULT ENTERITIS COMPLEX (PEC)?

ANG NATURANG SAKIT AY KARANIWANG TUMATAMA SA TURKEY NA NAGIGING SANHI NG DIARRHEA, MABABANG TIMBANG AT NAGIGING DAHILAN DIN NG KAMATAYAN.

AYON KINA MICROBIOLOGISTERICA SPACKMAN AT DARRELL KAPCZYNSKI, ANG NATURANG GADGET AY MAY KAKAYANANG MATUKOY ANG IBAT-IBANG URI NG VIRUS SA ISANG PAGKAKATAON LAMANG.

NAKASALALAY ANG DIAGNOSTIC TEST NA ITO SA MOLECULAR TECHNIQUE NA TINATAWAG NA REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RRT-PCR) NA MAITUTURING NA HIGHLY SENSITIVE AT SPECIFIC METHOD PARA MATUKOY ANG VIRAL RNA.

MALAYONG MAS MAGALING ITO KESA SA KASALUKUYANG PAMAMARAAN NG DIAGNOSTIC METHOD NA MERONG LIMITASYON DAHIL SA POOR ANALYTIC SPECIFICITY AT SENSITIVITY.

ISINAILALIM SA INOCULATION ANG TURKEY POULTS NG PEC-ASSOCIATED VIRUS AT SAKA KINOLEKTA ANG INTESTINAL TISSUE SAMPLES AT CLOACAL SWABS MULA SA EXPERIMENTAL INFECT BIRDS.

NAIPAKITA NG RRT-PCR TEST ANG HIGH SENSITIVITY, AT TUMPAK NA DETECTION NG TARGET VIRUS.

0 Comments:

Post a Comment

<< Home