Wednesday, September 20, 2006

Gene Delivery Truck Aids Non-Chemical Pest Control

Gene "Delivery Truck" Aids Nonchemical Pest Control

In a pest-control approach called the sterile insect technique, irradiation is used to sterilize factory-grown males of a pest insect, like screwworms or Mediterranean fruit flies.

When released into the field, the males mate with wild females and no offspring are produced, thus eliminating or reducing the need for chemical insecticides.Over the past several years, Agricultural Research Service (ARS) scientists have conducted research using molecular genetics to develop genetic systems to sterilize pest insects instead of irradiating them.

This new approach calls for using modified pieces of DNA, called transposons, to introduce foreign genes that can cause sterility in pest insects.The trick is making sure the transposons don't move after they're inserted into the insect's chromosomes.

Otherwise, the sterilizing trait can be lost, notes Paul Shirk, a physiologist with ARS' Center for Medical, Agricultural and Veterinary Entomology in Gainesville, Fla.

As an alternative to using the transposons, Shirk and colleagues disabled the machinery of a virus that infects the common buckeye butterfly and turned the pathogen into a gene "delivery truck" called JcDNV.

When injected into an insect's embryonic stage, the JcDNV delivers its load--a foreign gene--into a chromosome.

The gene stays put, and scientists can monitor its activity throughout the insect's life, including whether it passes the gene to offspring.However, unlike the transposons, the JcDNV restricts its deliveries to the insect's somatic (body) cells, but not its reproductive cells, notes Shirk, who's in the ARS center's Insect Behavior and Biocontrol Research Unit.

Shirk collaborated with Max Bergoin, a virologist who originally cloned the virus, Junonia coenia densovirus, at the University of Montpellier in France, and a postdoctoral team comprising Herve Bossin and Jennifer Gillett (both formerly ARS), and ARS technician Richard Furlong.

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AGRI-INFO/SEPTEMBER 20, 2006


TITLE: GENE DELIVERY TRUCK AIDS NON-CHEMICAL PEST CONTROL


ALAM BA NINYO NA SA PAGPUKSA NG PESTE NA TINAGURIANG STERILE INSECT TECHNIQUE, GINAGAMIT ANG IRRADIATION PARA MA-STERILIZE ANG MGA TINATAWAG NA FACTORY-GROWN MALES NG ISANG URI NG PESTENG INSEKTO, KABILANG NA RITO ANG SCREWWORMS O KAYA AY MEDITTERANEAN FRUIT FLIES.

KAPAG PINAKAWALAN SA LUPANG SAKAHAN, ANG MGA LALAKENG PESTE AY NAKIKIPAGNIIG SA WILD FEMALES AT WALA SILANG MAGIGING SUPLING DAHILAN PARA MAIBSAN KUNDI MAN MAWALA NG TULUYAN ANG MGA KALABANG INSEKTO NGUNIT MALAKING KATIPIRAN SA PAGGAMIT NG KEMIKAL.

SA MGA NAKALIPAS NA MARAMING TAON, AY NAGSAGAWA NG MALALIMANG PANANALIKSIK TUNGKOL DITO ANG MGA EKSPERTO SA PAMAMAGITAN NG PAGGAMIT NG TINATAWAG NA MOLECULAR GENETICS PARA MAISAKATUPARAN ANG GENETIC SYSTEMS AT MA-STERILIZE ANG MGA PESTENG INSEKTO AT ISAILALIM SILA SA IRRADIATION.

ANG BAGONG PAMAMARAAN NA ITO AY NANGANGAILANGAN NG MODIFIED PIECES NG DNA NA TINATAWAG NA TRANSPOSONS, PARA MAGAMIT ANG FOREIGN GENES NA SIYANG SANHI NG STERILITY SA MGA PESTE.

KAILANGAN LAMANG NA TIYAKIN NA HINDI GAGALAW ANG TRANSPOSONS NA ITO HABANG SILA AY ISINISINGIT SA CHROSOMOSOME NG MGA INSEKTO.

KUNDI MAN AY NAGBABALA SI PAUL SHIRK, PHYSIOLOGIST NG ARS CENTER FOE MEDICAL, AGRICULTURAL AT VETERINARY ENTOMOLOGY NA KAPAG DI NASUNOD ANG TAMANG PROSESO AY POSIBLENG MAWALAN NG SAYSAY ANG STERILIZING POWER.

1 Comments:

At 6:52 AM, Blogger Sasha said...

this is a very insightful information. I have encountered non-chemical pest controls in the past and I was wondering if this same non-chemical content will work on removing earwigs in my garden?

I have also heard the cayene pepper will correct the infestation.. any suggestions? = earwig infestation

 

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